Chemical Composition and Antioxidant Potential of Ethylacetate Leaf Extract of (Gossypium hirsutum) Linn
Published: 2024-07-11
Page: 156-168
Issue: 2024 - Volume 7 [Issue 2]
Ogunmola Oluranti Olagoke *
Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria and Department of Physical Sciences Education, Emmanuel Alayande University of Education, Oyo, Nigeria.
Adedosu Taofik Adewale
Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Majolagbe Olusola Nathaniel
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Amusat Mumini Adekunle
Department of Biology and Integrated Science, Emmanuel Alayande University of Education, Oyo, Nigeria.
Azeez Eniola Morufat
Department of Biology and Integrated Science, Emmanuel Alayande University of Education, Oyo, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Gossypium hirsutum is an important plant with therapeutic properties; hence this study seeks to screen the leaf extract for its chemical composition and antioxidant activities in order to find possible natural sources of novel phytochemicals in pharmaceutical formulations. The qualitative and quantitative phytochemical screening was conducted using standard procedure, the chemical composition was determined using gas chromatography mass spectrophotometry (GC-MS) while the antioxidant activities was determined using three assays, DPPH, ABTS and NOS. the qualitative phytochemical screening revealed the presence of saponin, alkaloid, phenol, tannin, phytate, steroids, terpenoids, flavonoids and proanthocyanidin. Alkaloid was conspicuously absent in the extract. The quatitative phytochemical screening revealed the presence of saponins, flavonoids, phenols, steroids, phytates, terpenoids and proanthocyanidins in different concentration in which total phenol is the highest 552.64±35.30 mg/100g of the extract while total saponin is the lowest 22.25±1.41 mg/100g of the extract. The GC/MS analysis revealed the presence of 24 compounds which include caryophyllene with the highest percentage composition 33.62%. The antioxidant activities of the extract revealed that the extract had a moderate antioxidant activities with EC50 of 12.12, 6.01 and 5.01 mg/mL. In conclusion, the leaf extract demonstrated antioxidant properties suitable for exploration in new drug development.
Keywords: Gossypium hirsutum, phytochemical, antioxidant
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Nagy N. Phytochemical Analysis of Biologically Active Constituents of Medicinal Plants. Journal of Main Group Chemistry. 2014;13:21-28.
Mukherjee PK, Wahile A. Integrated approaches towards drug development from ayuveda and other Indian system of medicine. Journal of Ethnopharmacology. 2006;103:25-35.
Chan BG, waiss Jr AC, Lukefahr MJ. Condensed tannin an antibiotic Chemical from Gossypium hirsutum. Journal of Insect Physiology. 1978;(24):113-118.
Shidaifat F. Inhibition of human postrate cancer cells growth by gossypol associated with stimulation of transforming growth factor-beta. Cancer Letter. 1996;107(1):37-44.
Salako OA, Awodele O. Evaluation of the antimalaria activity of the aqueous leaf extract of Gossypium barbadense (Malvaeceae) in mice. Drugs and Therapy Studies. 2012;2(2):1-7.
Ajayiyeoba EO, Falade CO, Fawole OI, Akinboye DO, Gbotosho GO, Bolaji OM, et al. Efficacy fof herbal remedies used by herbalist in Oyo State Nigeria for treatment of Plasmodium falciparum infections- a survey and observation. African Journal of Medicinal Science. 2004;33(2):115- 199.
Larayetan R, Ojemaye MO, Okoh OO, Okoh AL. Silver Nanoparticles Mediated by Callistemon citrinus Extracts and their Antimalaria, Antitrypanosoma and Antibacterial Efficacy. Journal of Moelcular Liquids. 2019;273:615-25.
Pandey A, Tripathi S. Concept of Standardization, Extraction and Pre-Phytochemical Screening Strategies for Herbal Drug. Journal of Pharmacognosy and Phytochemistry. 2014;2:115-119.
Kim BH, Hackett MJ, Park J, Hyeon T. Synthesis, characterization, application of ultra-small nanoparticles. Chemical Mater. 2014;26:59–71.
Ordone Z, Gomez AAL, Vattuno JD, MA, Isla MI. Antioxidant activities of Sechium edule (Jacq). Swarts extracts. Food Production. 2006;97(3):452–458.
Van Buren JP, Robinson WB. Formation of Complexes between Protein and Tannic Acid. Journal of Agriculture and Food Chemistry. 1969;17(4):772-777.
Iwu IC, Onu U, Adanma U. Phytochemical Antimicrobial and GC/MS Analysis of the Root of Stachytapheta cayennesis (L. Vahl) Grown in Eastern Nigeria. Journal of Natural Sciences Research. 2018;6(2):1-14.
Harland BF, Oberleas D. A Modified Method for Phytate Analysis Using an Ion Exchange Procedure: Application to Textured Vegetable Protein. Cereal Chemistry. 1977;54(4):827-832.
AOAC. Official Methods of Analysis of AOAC International; 2005.
Iwu IC, Chiojioke OM, Onu UL, Uchegbu R. GC-MS Phytochemical and Antimicrobial Analysis of the Leaf of Newboudia leavis P. Benth. International Journal of Innovative Research and Development. 2018;7(7):242-250.
Trease G, Evans SM. Pharmacognosy. 15ed. London: Bailer Tindal. 2002;23-67.
Sun Y, Xia Y. Gold and Silver Nanoparticles: A Class of Chromophores with Colors tunable in the range from 400 to 750 nm. Analyst. 2003;128(6):686-91.
Larayetan R, Ojemaye MO, Okoh OO, Okoh AI. Silver nanoparticles mediated by Callistemon citrinus extracts and their antimalaria, antitrypanosoma and antibacterial efficacy. Journal of Molecular Liquids. 2019;1(273):615-25.
Larayetan RA, Okoh OO, Sadimenko A. Terpene Constituents of the Aerial Parts, Phenolic Content, Antibacterial Potential, Free Radical Scavenging and Antioxidant Activity of Callistemon citrinus (Curtis) Skeels (Myrtaceae) from Eastern Cape Province of South Africa. BMC Complementary and Alternative Medicine. 2017;17:292. Available:https://doi.org/10.1186/s12906-017-1804-2
Pal M, Misra K, Dhillon G, Brar SK, Verma M. Antioxidants In P. Cordon (Ed.), Biotransformation of waste Biomass into High Value Biochemicals, Oxford, Elsevier. 2014;117-138.
Omojasola PE, Awe S. The Antibacterial Activity of the Leaf Extracts of Anarcadium occidentale and Gossypium hirsutum against Selected Micro-organisms. Bioscience Resources Communications. 2004;16(1):25–28.
Ade-Ademilua OE, Okpoma MO. Gossypium hirsutum L, Gossypium barbadense L: Differences in Phytochemical Content, Antioxidant and Antimicrobial Properties. Ife Journal of Science. 2018;20(1):77–88.
Jokotagba OA, Onasanya SS, Siyanbola TO. Phytochemical and GcMs Analyses of Medicinal Properties of Ethanol Extract of Gossypium barbadense Leaves. YABATECH International Journal of Science and Society. 2016;4(1):47–52.
Okudu T, Yoshida T, Hatano T. Food phytochemicals forcancer prevention II. In:Ho C. T, Osawa T, Huang M. T, Rosen R. T. (Eds.) Chemistry and Antioxidative Effects of Phenolic Compounds from Licorice, Tea and Compositae and Labiateae Herbs. ACS, Washington. 1994;132-143.
Tong Y, Qi L, Stefenelli G, Wang DS, Canonala F, Baltensperger U, Prevot ASH, Slowik JG. Quantification of Primary and Secondary organic aerosol by combined; 2022. Available:https://amt.copernicu.org>2022
Muhammad Z, Atabo S, Zakari A. Characterization of bioactive components of Gossypium barbadense L. with hematinic potential in Wister albino rats. British Journal of Pharmaceutical Research. 2014;4(21):2563.
Shi L, Arntfield SD, Nickerson M. Changes in levels of phytic acid, lectins and oxalates during soaking and cooking of Canadian pulses. Food Research International. 2018;107(2017):660–668. Available:https://doi.org/10.1016/j.foodres.2018.02.056
Franklin LU, Cunnington GD, Young DE. Terpene based Pesticide Treatment for Killing Terrestrial Anthropods Including, amongst others, Lice, Lice Eggs, Mites and Ants. US Patient. 2000;6(130):253– 260.
Guimaraes AC, Meireless LM, Lemos MF, Guimaraes MCC, Endringer DC, Fronza M, Scherer R. Antibacterial Activity of Terpenes and Terpenoids Present in Essential Oil. Molecules. 2019;24(13): 2471. Available:https:// doi.org/10.3390/molecules 24132471
Gary B. Proanthocyanidins: Biological Activities Associated with Human Health. Archives of physiology and biochemistry. 2004;42:2-20. DOI: 10.1080/13880200490893474.
Koes R, Verweij W, Quattrocchio F. Flavonoids: A colorful model for the regulation and evolution of biochemical pathways. Trends in Plant Science. 2005;10(5):236-242.
Yao LH, Jiang YM, Shi J, et al. Flavonoids in Food and Their Health Benefits. Plant Foods Hum Nutr. 2004;59:113– 122. Available:https://doi.org/10.1007/s11130-004-0049-7
Crozier A, Jaganath IB, Clifford MN. Phenols, polyphenols and tannins: An overview. Plant secondary metabolites: Occurrence, structure and role in the human diet; 2006. DOI:10.1002/9780470988558. Ch1.
Francomano F, Caruso A, Barbarossa A, Fazio A, La Torre C, Caramella J, et al. β-caryophellene: A susquiterpene with countless Biological properties. Applied Science. 2019;9(24):5420
Hashish H, Rahiman NMMF, Sharma C, Sadek BS. Therapeutic Potential of β-caryophellene: A dietary cannabinoid in Diabetes and Associated Complications. Nutrients. 2020;12(10):2963
Ortega AMM, Campos MRS. Macronutrients and micronutrients in cancer prevention and treatment in (eds.) Oncological Functional Nutrition: Phytochemical and plants, Cambridge, Academic Press. 2021;99–124.
Vasudevan, Aparna, Vijayan, Dileep, Mandal, Pradeep, Karthe, Ponnuraj, Sadasivan, Chittalakkottu, Haridas, M. Anti-Inflammatory Property of n-Hexadecanoic Acid: Structural Evidence and Kinetic Assessment. Chemical Biology and Drug Design. 2012;80:434-9. DOI: 10.1111/j.1747-0285.2012.01418.x
Fernandes ES, Passos GF, Medeiros K, Cunha FM, Ferreira J, Campos MM, Pianowski LF, Calixto JB. Anti Inflammatory Effects of compounds Alpha-humulene and (-) trans-cary-Phyllene Isolated from the Essential Oil of Cardi Verbenacea. European Journal of Pharmacology. 2007;569:228–236.
Rogerio AP, Rade EL, Leite DF, Figueiredo CP, Calixto JB. Preventive and Therapeutic, Anti-Inflammatory Properties of the Sesquiterpene alpha-hoolene In Experimental air ways Allergic Inflammation. Britain Journal of Pharmacology. 2009;158 (4):1074–1087.
Satso H, Matsuda H, Toshimitso T, Mori A, Mae T, Tsukagawa M, Kitahara M, Shimizu M. Regulation of Interleukin–Secretion in human Intestinal epithelial caco–2 cells by α–humulene. Biofactors. 2004;21(1-4):137–139.
Gonzalez-Rivera ML, Barragan-Galvez JC, Gasca-Martinez D, Hidalgo-Figveroa S, Isiordia-Espinoza M, Alonso-castro AJ. In vivo Neuropharmacological effects of Neophytadiene. Molecules. 2023;28(8): 3457
Taj T, Sultana RS, Shahin H, Chakraborty Ahmed MG. Phytola Phyto constituent, Its Chemistry and Pharmacological Actions. GIS Science Journal. 2021;8(1):395– 406.
Schlesier K, Harwat M, Bohm V, Bitsch R. Assessment of Antioxidant Activity by using Different in vitro Methods. Free Radical Research. 2002;36(2):177- 187.
Manach C, Williamson G, Morand C, Salbert A, Remesy C. Bioavailability and Bio-efficacy of Polyphenols in Humans. I. Review of 97 Bioavailability Studies. The American Journal of Clinical Nutrition. 2005;81:230–242.
Sharima VK, Yngard RA, Lin Y. Silvener Nanoparticles: Green Synthesis and their Antimicrobial Activities. Advances in Colloid and Interface Science. 2009; 145:83–96.
Zhang Y, Luo Z, Wang D, He F, Li D. Phytochemical Profiles and Antioxidant and Antimicrobial Activities of the Leaves of Zanthoxylum bungeanum. Scientific World Journal. 2014;2014: PMC4134800.
Rayma MP. Selenium and Human Health. The Lancet. 2012;379(9822):1256- 1268.